Bacstain- Bacterial Viabiliy Detection Kit-CFDA/PI

Bacstain- Bacterial Viability Detection Kit – CFDA/PI uses esterase activity of bacteria and bacterial membrane damage as an index. CFDA is hydrolyzed to carboxyfluorescein?which is fluorescent?by intracellular esterase. PI is a parallel intercalator into the DNA double helix that stains nucleic acids; it passes only through damaged bacterial membranes. Thus, this kit can be used to measure the ratio of bacteria with esterase activity to membrane-damaged bacteria on the analysis of the fluorescent images from each stain.