For most soluble protein targets, the antibodies are generated by inoculating animals with purified, full-length protein. Unfortunately, this approach is not effective for most multiple-spanning membrane proteins, whose native structures normally depend on an intact lipid bilayer. G protein-coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, represent a large and important group of proteins involved in many diseases and are also the target of around half of all modern medicinal drugs.Unfortunately, it is extremely difficult to create antibodies against GPCRs using traditional approaches. Solubilization of the membrane protein immunogens with detergents may lead to major conformational changes; the epitopes are made up of small discontinuous segments. Moreover, these proteins in general are expressed at very low levels and it is difficult to generate sufficient material for immunization. Production of GPCRs antibodies remains a challenge.

  • Membrane Protein Antibody
  • Antibody Production

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