ILS INNOVATIVE LABOR SYSTEME GMBH

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Plunger Force Adjustment Video Tutorial ILS glass syringes are manufactured to exacting standards and are quality tested to meet or exceed our minimum performance specifications for accuracy and precision. Our testing methods are in accordance GLP protocols and utilize equipment that is traceable to NIST standards. Copies of actual test result are available upon request. Syringe use: - eliminate the carry over between the samples. Rinse the syringe with the to be examined liquid 10-20 times. Wash the syringe and discard the first 2 -3 washes. - for injection overfill the microlitersyringe. Press the plunger until the accurate volume. Syringe cleaning - pull the piston out of the glass body of the microsyringe - rinse inside the glass body with detergents (in the final rinsing cycle it is best to use a low boiling point, solvent or alcohol) - dry the glass body by air flow, e.g. with a vacuum pump CAUTION: DO NOT IMMERSE THE MICROSYRINGE IN THE SOLVENT: THE GLUE PARTS CAN BE SOLVED. Needle blocked: - use the cleaning wires from ILS you find a wide range at www.microsyringes.com Plunger with seal - if you want reduce the plunger force, heat the assembled syringe in oven at 60°C 15 minutes - Silicone or other lubricants that are compatible with your sample may also be used to wet the plunger tips CAUTION: RUNNING A TEFLON-TIPPED PLUNGER DRY MAY CAUSE THE PTFE TO SHRED OR PULL LOOSE FROM THE PLUNGER ROD, RESULTING IN LEAKS. Plunger Tip ss*=stainless steel (N- Series) -clean the plunger in an ultra sonic bath - wipe the piston with a fibre-free cloth (i.e. no paper towel) Plunger of zero dead volume syringes (T- Series) - loose the nut before pull out or insert the plunger - wipe the piston with a fibre-free cloth (i.e. not paper towel)

Glass Barrel: Borosilicate 3.3 Precision: < ±1 % of the volume Main use: - sample dosage for gas chromatography (GC) - HPLC-valves - autosamplers - Thin Layer Chromatography (TLC) Fixed Needle (FN) not autoclavable stainless steel= ss* Needle Tip Needle Tip a a b c d a (FN) (FN) (FN) (FN) (FN) (FN) Length 51 mm 70 mm 51 mm 51mm 51 mm 51 mm Plunger Part Part Part Part Part Part Volume Tip O.D.(Gauge) No. No. No. No. No. No. Six-Pack 5µl ss* 0.47(26s) 2100301 - 2100302 2100303 2100304 2100311 10µl ss* 0.47(26s) 2100401 2100409 2100402 2100403 2100404 2100411 25µl ss* 0.72(22s) 2100501 2100509 2100502 2100503 2100504 - 50µl ss* 0.72(22s) 2100601 2100609 2100602 2100603 2100604 - 100µl ss* 0.72(22s) 2100701 2100709 2100702 2100703 2100704 - 250µl ss* 0.72(22) 2100801 - 2100802 2100803 2100804 - 500µl ss* 0.72(22) 2100901 - 2100902 2100903 2100904 - Series N - Fixed Needle(FN), with guide Needle Tip a a; b; c Length 51mm Plunger Part Please specify with order: Volume Tip O.D.(Gauge) No. Needle Tip &amp; Length (in mm) 5µl ss* 0.47(26s) 2170301 2110300 10µl ss* 0.47(26s) 2170401 2110400 25µl ss* 0.72(22s) 2170501 2110500 50µl ss* 0.72(22s) 2170601 2110600 100µl ss* 0.72(22s) 2170701 2110700 250µl ss* 0.72(22) 2170801 2110800 500µl ss* 0.72(22) 2170901 2110900 80366 80383 80400 80439 80500 80539 80639 80600 80700 80739 80800 80839 208546 208646 002003 002050 036410 003050 038410 004000 004050 038410 006000 87939 87900 005000 87919

Glass Barrel: Borosilicate 3.3 Precision: < ±1 % of the volume Main Use: - sample dosage for gas chromatography (GC) - for autosamplers - for HPLC-valves - Thin Layer Chromatography (TLC) Removable Needle (RN) autoclavable stainless steel= ss* Needle Tip Needle Tip a b a (RN) (RN) (RN) Length 51 mm 51 mm 51 mm Plunger Part Part Part Volume Tip O.D. (Gauge) No. No. No. 5µl ss* 0.47(26s) 2100305 2100306 - 10µl ss* 0.47(26s) 2100405 2100406 2100415 25µl ss* 0.72(22s) 2100505 2100506 - 50µl ss* 0.72(22s) 2100605 2100606 - 100µl ss* 0.72(22s) 2100705 2100706 - 250µl ss* 0.72(22) 2100805 2100806 - 500µl ss* 0.72(22) 2100905 2100906 - Series N - Removable Needles(RN), with Guide Needle Tip a a; b; c (RN) (RN) Length 51mm Please specify Volume O.D.(Gauge) with Guide Needle Tip &amp; Length (in mm) 5µl 0.47(26s) 2170305 2110301 10µl 0.47(26s) 2170405 2110401 25µl 0.72(22s) 2170505 2110501 50µl 0.72(22s) 2170605 2110601 100µl 0.72(22s) 2170705 2110701 250µl 0.72(22) 2170805 2110801 500µl 0.72(22) 2170905 2110901 guide GUIDE 80430 80530 80830 87930 004050 005050 006050

Glass Barrel: Borosilicate 3.3 Precision: < ±1 % of the volume Main Use: - sample dosage stainless steel= ss* Plunger Part Volume Tip No. 50µl ss* 2100611 100µl ss* 2100711 250µl ss* 2100811 500µl ss* 2100911 80520 80620 80720 80820

Glass Barrel: Borosilicate 3.3 Precision: < ±1 % of the volume Main Use: - sample dosage - Syringes are autoclavable when disassembled stainless steel= ss* Plunger Part Volume Tip No. 5µl ss* 2100312 10µl ss* 2100412 25µl ss* 2100512 50µl ss* 2100612 100µl ss* 2100714 250µl ss* 2100812 500µl ss* 2100912 80301 80401 80501 80601 80701 80801

Glass Barrel: Borosilicate 3.3 Precision: < ±1 % of the volume Main Use: - sample dosage for gas chromatography - HPLC-valves - autosamplers Plunger: - Nickel titanium alloy, flexible memory metal Removable Needle (RN) autoclavable Fixed Needles (FN) not autoclavable Needle Tip Needle Tip a b c d (FN) (FN) (FN) (FN) Length 51 mm 51mm 51 mm 51 mm Part Part Part Part Volume O.D. (Gauge) No. No. No. No. 5µl 0.47(26s) 2160301 2160302 2160303 2160304 10µl 0.47(26s) 2160401 2160402 2160403 2160404 Needle Tip a b c d (RN) (RN) (RN) (RN) Length 51 mm 51mm 51 mm 51 mm Part Part Part Part Volume O.D. (Gauge) No. No. No. No. 5µl 0.47(26s) 2160305 2160306 2160307 2160308 10µl 0.47(26s) 2160405 2160406 2160407 2160408

Glass Barrel: Borosilicate 3.3 Precision: < ±1 % of the volume Main Use: - sample dosage for gas chromatography - dosage of small amounts of liquids - for Thin Layer Chromatography (TLC) Removable Needles (RN) autoclavable stainless steel= ss* plunger runs inside the needle (plunger in needle) Needle Tip Tip Style a b c d (RN) (RN) (RN) (RN) Length 70mm 70mm 70mm 70mm Plunger Part Part Part Part Volume Tip O.D.(Gauge) No. No. No. No. 0.5µl ss* 0.47(26s) 2200051 2200058 - - 0.5µl ss* 0.64(23) 2200062 2200056 2200061 - 1µl ss* 0.47(26s) 2200101 2200102 - 2200103 1µl ss* 0.64(23) - - 2200113 2200303 2µl ss* 0.72(22s) 2200201 2200202 - - 5µl ss* 0.55(24) 2200301 2200302 - 2200203 10µl ss* 0.72(22s) 2200401 2200402 - 2200403 25µl ss* 1.10(19) 2200501 2200502 - 2200503

Plunger: Glass barrel: Borosilicate 3.3 Precision: < ±1 % of the volume Main Use: - sample dosage for gas chromatography - dosage of small amounts of liquids - for Thin Layer Chromatography (TLC) Replacement Kit Includes 1 needle and 1 calibrated plunger Stainless steel= ss* plunger runs inside the needle (plunger in needle) Needle Tip Tip Style a a b b (RN) (RN) (RN) (RN) Sealing Kit Length 70mm 70mm 70mm 70mm Includes 5 seal Plunger Part Part Part Part Part Volume Tip O.D.(Gauge) No. No. No. No. No. 1µl ss* 0.47(26s) 2231101 - 2231102 - 2230001 2µl ss* 0.72(22s) - 2231201 - 2231202 2230002 5µl ss* 0.55(24) 2231301 - 2231302 - 2230003 10µl ss* 0.70(22s) - 2231401 - 2231402 2230004 25µl ss* 1.10(19) - 2231501 - 2231502 2230005

Glass barrel: Borosilicate 3.3 Precision: < ±1 % of the volume Main Use: - sample dosage for gas chromatography (GC) - HPLC-valves - autosamplers - dosage of aggressive fluids Fixed Needle (FN) not autoclavable Needle Tip Tip Style a b c d a (FN) (FN) (FN) (FN) (FN) Length 51mm 51mm 51mm 51mm Plunger Part Part Part Part Part Volume Tip O.D.(Gauge) No. No. No. No. No. 6-Pack 5µl PTFE 0.47(26s) 2600301 2600302 2600303 2600304 2600311 10µl PTFE 0.47(26s) 2600401 2600402 2600403 2600404 2600411 25µl PTFE 0.72(22s) 2600501 2600502 2600503 2600504 - 50µl PTFE 0.72(22s) 2600601 2600602 2600603 2600604 - 100µl PTFE 0.72(22s) 2600701 2600702 2600703 2600704 - 250µl PTFE 0.72(22) 2600801 2600802 2600803 2600804 - 500µl PTFE 0.72(22) 2600901 2600902 2600903 2600904 - 1.0ml PTFE 0.72(22) 2600000 2600001 2600016 2600002 - 1.0ml PTFE 0.64(23) 2600003 - - 2600008 - 2.5ml PTFE 0.64(23) - - 2600037 2600028 - 2.5ml PTFE 0.72(22) 2600020 2600021 - 2600022 - 5.0ml PTFE 0.72(22) 2600040 2600041 - 2600042 - 10.0ml PTFE 0.72(22) 2600060 2600061 - 2600062 - Series H - Fixed Needles, Customized Length Needle Tip a; b; c Length Please specify Plunger Needle Tip &amp; Length (in mm) Volume Tip O.D.(Gauge) Art.No. 5µl PTFE 0.47(26s) 2610300 10µl PTFE 0.47(26s) 2610400 25µl PTFE 0.72(22S) 2610500 50µl PTFE 0.72(22s) 2610600 100µl PTFE 0.72(22s) 2610700 250µl PTFE 0.72(22) 2610800 500µl PTFE 0.72(22) 2610900 1.0ml PTFE 0.72(22) 2610000 2.5ml PTFE 0.72(22) 2610020 5.0ml PTFE 0.72(22) 2610040 10.0ml PTFE 0.72(22) 2610060 80000 80200 80039 80075 80339 80350 80900 80939 91000 81039 81100 81139 81417 81516 81517 81616 81617 81643 003200 003250+038410 004200 005200 002250+037410 81316 81317 81416