APPLICHROM GMBH

AppliChrom ABOA SuperOH-P - optimized for aqueous GPC/SEC-separations - aqueous high hydrophilic polymeric base material - low column bleeding for low detector noise - 7μ particle technology (standard) for high platecounts and high resolution*) - large pore volume for high resolution - pH stable 2.5-12 - pressure stability 30-80bar (depending on poresize) - temperaturestability 10-85°C - individual poresizes for individual molecular weight ranges - multi-pore technology for broad range of molecular size - increased livetime of GPC/SEC columns by combination of proprietary particle- and packing technology service-applicationcenter for methodscreening available in Oranienburg (Germany / Europe)

AppliChrom GPC-columns for GPC analysis of organic molecules using (THF, toluene, chloroform)**. Oligomers and polymers including the new GPC media line from AppliChrom BPT* synthesis technology for large range, high resolution separations with increased accuracy in calibration - spherical high porous styrene-divinylbenzene GPC-media - large molecular weight range: 100 10.000.000Da - optimized for 1ml/min flowrate when using 8mm ID-columns - high pressure stability of 150-50bar, depending on porosity - high capacity from AppliChrom 8mm ID GPC columns (5% more capacity than 7,8mm columns, 14% more capacity than 7,5mm ID columns) - plus extra high pore volume from AppliChrom GPC synthesis technology for an extra increasing of peak capacity and resolution - proprietary AppliChrom GPC column packing procedure for accurate peak performance, low back pressures and an extension of column livetime

For HPLC-Analyses of - Sugars/Carbohydrates - Sugar Alcohols - Alcohols - Carboxylic Acids

For GPC / SEC analysis in DMSO, examples: - amylose, amylopectin, starch - urea-formaldehyd resins (UF-resins) - melamin-urea-formaldehyd resins (MUF-resins) - lignins, humic substances, humic acids, coniferous wood bark essences - polysaccharide, polysaccharid derivatives - poly(N-isopropylacrylamid) PNIPA - poly-vinylpyridin - calibration: pullulan, dextran, polyvinylpyridin et al. Advantages - optimized for DMSO-GPC applications - interactionfree pure GPC/SEC - easy, reliable and robust GPC/SEC-calibration by dextrans, pullulans et al. - low column bleeding for low detectornoise for improved lightscattering or viscosity detection - 12μ particle technology for low backpressure - large porevolume and optimized mass transfer for polymers giving optimized resolution - low costs caused by long livetime of column – result of combination of optimized proprietary particle and packingtechnology.

Special GPC / SEC columns for the aqueous analysis of neutral, anionic and additionally cationic polymers. For GPC / SEC analysis of - Polycations, polyammonium compounds - polyamines (chitosans), polyethylenimines, - PEGylated polyethyleneimines - polysaccharides - polyanions (heparins, pectins, ...)

AppliChrom GPC columns for GPC analyzes organic (DMAc, DMF and NMP) Spherical porous polymeric GPC chromatography phases - wide range of pore and particle sizes - optimal for 0.5ml / min flow rate with 8mm ID columns (40-50°C) - Pressure stability 50-150bar, depending on the porosity - high resolution due to high pore volume - long service life - high reproducibility - high purity of the GPC column for good interaction-free GPC - Please avoid: drying out - Molar mass range: 100-1,000,000Da - Examples: PMMA, PAN, cellulose, DMF and DMAc soluble polymers

Polymeric Size Exclusion Chromatography Columns for high Resolution and Recovery in Protein Analysis/Purification.